Catalog No: CY5528 Reactivity: Human Mouse Rat
Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP FC
UniProt ID: Q9H492
All Names: MAP1LC3;LC3;LC3A;MAP1ALC3;MAP1BLC3 ;MAP1LC3A;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 16 kDa
WB: 1:500~1:2000
IHC: 1:50~1:200
ICC/IF: 1:50~1:200
IP: 1:50
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated
proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein
Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational
modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following
synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by
a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic
vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form
LC3-II have been used as indicators of autophagy.

Western blot analysis of MAP1LC3A expression in Hela cell lysate.