Catalog No: AB0061 Reactivity: Human , Mouse , Monkey , Goat , Rat , Hamster , Saccharomyces cerevisiae , Arabidopsis , Pichia pastoris , Rabbit , Zebrafish , Drosophila , Leaf of Rice , Fruit of Cucumber , Chlamydomonas Reinhardtii
Isotype: Mouse IgG2b Applications: WB
UniProt ID: P60709
All Names: ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 42kDa
WB 1:3000~1:10000
beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

Western blot analysis of beta Actin expression in
(1) Hela;
(2)Human fetal kidney lysate;
(3) 3T3 cell lysate;
(4) PC-12 cell lysate;
(5) COS-1 cell lysate;
(6) Goat muscle lysate.